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Figure 3: Comparative full field optical coherence tomography and H and E-stained histology (a) Testis of a normal rat shows seminiferous tubules with relatively uniform size and shape (b) H and E histology stain of the same specimen. Arrows point to the sperm within the tubule lumen. (c) Seminiferous tubules in the testis of a rat treated with busulfan, showing thinner tubules and a greater degree of heterogeneity in size and shape with ~10% normal spermatogenesis. (d) H and E staining of the same specimen. Field of view in each panel: 1 mm2 Permission granted under the creative commons attribution license[11]

Figure 3: Comparative full field optical coherence tomography and H and E-stained histology (a) Testis of a normal rat shows seminiferous tubules with relatively uniform size and shape (b) H and E histology stain of the same specimen. Arrows point to the sperm within the tubule lumen. (c) Seminiferous tubules in the testis of a rat treated with busulfan, showing thinner tubules and a greater degree of heterogeneity in size and shape with ~10% normal spermatogenesis. (d) H and E staining of the same specimen. Field of view in each panel: 1 mm2 Permission granted under the creative commons attribution license[11]