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Figure 2: Immunohistochemical staining with Bcl-2, caspase-3 and Bax (250) of rats fed normal diet (a), zinc deficient diet (b) and zinc supplementation diet (c). These staining reactions show the following: *Intense staining with Bcl-2 of mature spermatozoa *Intense staining with Bax (black) and caspase-3 (red) consistent with increased apoptosis of the early germ cells such as spermatogonia, spermatocytes and spermatids associated with zinc deficiency *intense staining with Bcl-2 of mature spermatozoa in the seminiferous tubule of rats fed with zinc supplementation diet

Figure 2: Immunohistochemical staining with Bcl-2, caspase-3 and Bax (250) of rats fed normal diet (a), zinc deficient diet (b) and zinc supplementation diet (c). These staining reactions show the following: *Intense staining with Bcl-2 of mature spermatozoa *Intense staining with Bax (black) and caspase-3 (red) consistent with increased apoptosis of the early germ cells such as spermatogonia, spermatocytes and spermatids associated with zinc deficiency *intense staining with Bcl-2 of mature spermatozoa in the seminiferous tubule of rats fed with zinc supplementation diet