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Figure 4: Assessment of apoptosis by Annexin V/PI on human prostate cancer cells (PC-3). The cells were treated with 10 and 20 ƒÝg/ml safranal for 48 h (symbol II, III) or media only (control symbol I), and apoptosis was examined by fl ow cytometry after Annexin V/PI double staining. Necrotic cells lose membrane integrity, permitting PI entry. Viable cells exhibit Annexin V (−)/PI (−); early apoptotic cells exhibit Annexin (+)/PI (−); late apoptotic cells or necrotic cells exhibit Annexin V (+)/PI (+)

Figure 4: Assessment of apoptosis by Annexin V/PI on human prostate cancer cells (PC-3). The cells were treated with 10 and 20 ƒÝg/ml safranal for 48 h (symbol II, III) or media only (control symbol I), and apoptosis was examined by fl ow cytometry after Annexin V/PI double staining. Necrotic cells lose membrane integrity, permitting PI entry. Viable cells exhibit Annexin V (−)/PI (−); early apoptotic cells exhibit Annexin (+)/PI (−); late apoptotic cells or necrotic cells exhibit Annexin V (+)/PI (+)