Indian Journal of Urology Users online:1345  
IJU
Home Current Issue Ahead of print Editorial Board Archives Symposia Guidelines Subscriptions Login 
Print this page  Email this page Small font sizeDefault font sizeIncrease font size


 
RESEARCH ARTICLE
Year : 2004  |  Volume : 20  |  Issue : 2  |  Page : 109-112
 

Effect of tropical vaginal hydroceles on testicular morphology and histology


1 Department of Surgery, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India
2 Department of Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India

Correspondence Address:
N Ananthakrishnan
Department of Surgery, JIPMER, Pondicherry - 605 006
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

 
   Abstract 

Objectives : Currently data on the effect of long stand­ing hydrocele on the testis is contradictory. A study was carried out to evaluate whether vaginal hydroceles affect testicular morphology andd histology.
Methods : Twenty-five patients with unilateral vaginal hydroceles were prospectively studied. The morphology and histology of the affected testis was studied and com­pared with the opposite unaffected testis.
Results : It was found that unilateral hydroceles did not affect ipsilateral scrotal temperature or the ipsilateral testicular volume. Histologically there was marked thick­ening of the tunica albugenia and the basement membrane on the affected side. There was distortion of the seminifer­ous tubules arrangement with marked intertubular edema. There was also significant reduction in the total seminif­erous cell count on the affected side due primarily to a decrease in spermatids. There was premature sloughing of the spermatogenic cells into the lumen of the tubules. .None of these changes correlated with the duration of the hydrocele, volume of the hydrocele fluid or intratunica vaginalis pressure.
Conclusions : It is concluded that vaginal hydroceles significantly affect the morphology of the testis by caus­ing reduction in the number of seminiferous tubules pri­marily spermatids besides producing changes in the structure and arrangement of the seminiferous tubules. It is not known whether these changes are reversible with treatment of the hydrocele.


Keywords: Hydrocele, testicular histology, sperm count, testosterone.


How to cite this article:
Srinath C, Ananthakrishnan N, Lakshmanan S, Kate V. Effect of tropical vaginal hydroceles on testicular morphology and histology. Indian J Urol 2004;20:109-12

How to cite this URL:
Srinath C, Ananthakrishnan N, Lakshmanan S, Kate V. Effect of tropical vaginal hydroceles on testicular morphology and histology. Indian J Urol [serial online] 2004 [cited 2019 Jun 27];20:109-12. Available from: http://www.indianjurol.com/text.asp?2004/20/2/109/21523



   Introduction Top


Vaginal hydroceles are common in young adults in tropi­cal countries particularly in areas endemic for lymphatic filariasis. Often these hydroceles are large and tense and exert considerable hydrostatic pressure on the enveloped testis. The intratunica vaginalis pressure frequently ex­ceeds venous pressure and it is likely that a large hydrocele may compromise the venous drainage from the ipsilateral testis. Simultaneously, stretching of the dartos muscle re­sults in loss of its contractility and thereby its ability to contract and alter the testicular position which is consid­ered important in the thermoregulation of the testis. It is likely therefore, that these combined effects of a tense vaginal hydrocele may affect testicular morphology and histology and perhaps its function. Such and effect, if proved, would strongly call, for early surgical treatment of vaginal hydroceles especially in young men.

This is especially important since WHO has embarked on a global programme on control of lymphatic filariasis, the twin pillars of which are mass drug administration to reduce transmission and morbidity management. This lat­ter particularly refers to lymphedema and hydrocele, which account for the bulk of the morbidity.

Studies that critically evaluate the effects of vaginal hydrocele on the testis are contradictory. These studies do not have controls or lack all necessary data such as size of hydrocele and intratunica pressure besides being qualita­tive and lacking in quantitative serological data. Some authors have found that hydroceles have no effect on testi­cular morphology [1],[2],[3] while others have described various changes. [4],[5] At present, the subject remains controversial. We, therefore, undertook to comprehensively evaluate tes­ticular morphology and histology in patients with unilateral vaginal hydroceles and to correlate the changes, if any, with the parameters such as duration of hydrocele, volume of hydrocele fluid and intratunica vaginalis pres­sure. The opposite unaffected testis served as a histologi­cal control.


   Patients and Methods Top


Twenty-five young men with unilateral vaginal hydro­celes were studied. A detailed history and clinical workup was done. All patients with associated inguinal hernia, varicocele, diabetes or history of mumps in childhood were excluded. Informed consent was obtained for testicular biopsy and ethical clearance was obtained from the Insti­tute's Research Council.

Scrotal skin temperature was measured on both sides of the scrotum separately with a digital thermometer be­fore shaving the part for surgery. All patients underwent eversion of the hydrocele sac on the affected side as the surgical treatment. The intratunica vaginalis pressure and the volume of the hydrocele fluid were measured at the time of surgery. The intratunica vaginalis pressure was measured using an 18 G needle, which was introduced into the hydrocele sac and connected to a manometer filled with saline. Readings were taken with the zero at the level of the pubic symphysis. The testicular length and width were measured with calipers after the testes were deliv­ered out through the incision in the scrotum, and the tes­ticular volume was computed using the formula, volume = π/6 LW 2 , where L is the length of the testis in cm and W the width. p was taken as 3.14. [6] A bilateral testicular bi­opsy with a strip of tunica albugenia measuring 6 x 4 mm was carried out. The tissue was fixed in Bouins's fluid and stained with hematoxylin and eosin.

Quantitative assessment of the testicular biopsy was carried out by point count using an eyepiece line grid. The proportionate volume of the seminiferous tubules, inter­tubular area and blood vessels was estimated using the stereological formula V 1 = P1 /Pt where, V 1 is the volume of that particular histological structure in question per cu­bic unit, P1 is the number of points against that particular histological structure and Pt is the total number of points in the line grid taken into consideration. [7]

The circumference of the seminiferous tubules was cal­culated using a micrometer for noting the longest (dl) and the shortest (d2) diameters of the seminiferous tubules in histological sections. Only the circular or more or less cir­cular tubules in the section were taken into consideration. The circumference was computed applying the formulae 2π(dl+d2)/4. [7] Twenty-five random seminiferous tubules were evaluated and the total number of seminiferous cells and percentage of spermatogonia, spermatocytes and spermatids were noted per unit circumference. A length of 1000 m was considered as a unit. The data collected was analyzed using the student t test. A probability value of 5% (p<0.05) was taken as significant. No attempt was made to study sperm counts or testicular endocrine func­tion as the study included only patients with unilateral hydrocele.


   Results Top


The age of patients ranged from 27-48 years (mean 36 years). The duration of hydroceles varies form 1-10 years (mean 4.1 years). The volume of the hydrocele fluid ranged form 200 to 1350 ml with a mean of 520 ml. Eleven of the 25 patients had fluid volumes in excess of 500 ml. The intratunica vaginalis pressure on the side of the hydrocele varied from 11 to 51 cm saline. In as many as 11 patients the pressure exceeded 30 cm of saline.

On an average the scrotal temperature was 3°C less than the axillary temperature and there was no significant dif­ference between the temperatures on the side of hydrocele as compared to the normal side (mean temperature on the affected side was 33.9±0.3°C and on the normal side was 33.9±0.4°C). The volume of the testis on the side of the hydrocele was larger than the opposite side in 17 patients and less than normal in the remaining eight (mean testicu­lar volume on the affected side was 24.0±11.6 ml and on the normal side it was 21.0±9.2 ml). This difference was not statistically significant.

Histological observations:

The tunica albugenia of the affected testis was mark­edly thickened in all patients. There was a distortion of the shape of the seminiferous tubules and the tubules were separated from each other [Figure - 1]. The basement mem­brane was thickened. Sertoli and Leydig cells were unaf­fected.

In addition, the testis on the side of the hydrocele showed marked sloughing of spermatogenic cells in division, i.e. pachytene, leptotene and diplotene type of spermatocytes and spermatids from the cells of Sertoli indicating prema­ture release of spermatogenic cells from seminiferous tu­bules. There was no interstitial fibrosis. These changes , are shown in [Figure - 1].

There was a significant increase in the proportionate volume of the intertubular area and significant reduction in the volume of the seminiferous tubules in the affected testis as compared to the control [Figure - 2] [Table - 1]. There was no significant difference seen for the volume occu­pied by blood vessels between the two sides showing thereby that the increased intertubular volume is due to venous dilatation consequent by the high intratunica pres­sure or due to proliferation of blood vessels.

The total seminiferous cell count per 1000 m circum­ference of seminiferous epithelium was significantly de­creased in the affected testis. It as 941.0+138.6 in the affected testis compared to 1106.4±192.1 within the nor­mal testis (p<0.01). The reduction in the germ cell counts in the affected testis was primarily due to reduction in the number of spermatids [Table - 2].

These histological changes did not correlate with the duration of hydrocele, the volume of hydrocele fluid or the intratunica vaginalis pressure [Table - 3].


   Discussion Top


Local pathophysiological alterations of the testicular milleu have a negative effect on fertility. Such effects have been demonstrated in patients with varicoceles. These include vari­ous degrees of oligospermia and histopathological changes such as decreased spermatogenesis. spermatogenic arrest at the primary spermatocyte stage, premature germ cell sloughing with abnormal forms, degeneration of the ad­luminal sertoli cell compartment, Leydig cell hyperplasia and small vessel fibrosis with luminal narrowing. [8],[9],[10] Such detailed studies on the effects of hydrocele on testis are scanty and contradictory.

In the present study, we found the testis in all patients to be of normal consistency with no evidence of atrophy. Testicular atrophy was found in 8% of patients reported by Dandapat et al. [4] The slight but insignificant increase in testicular size on affected side is probably due to thicken­ing of the tunica albugenia. The scrotal temperature was unchanged on the side of the hydrocele as reported ear­lier. [2]

Qualitatively, the present study showed that the tunica albugenia was thickened in all the patients. Dandapat et al reported this finding in 85% of their patients. Basement membrane thickening was seen in all our patients. This finding was observed in 25%, [1] 87% [11] and 78% [4] of patients in other studies. Interstitial fibrosis was not seen by us.  Sertoli cells More Details and Leydig cells were normal; a fact in agree­ment with other reports. [4] Quantitatively, there was an in­crease in the intertubular area due to interstitial edema. The mean germ cell count per unit circumference was signifi­cantly decreased in patients with hydrocele. This reduc­tion in germ cell count was primarily due to decrease in the spermatids, which are more sensitive to a vascular in­sult. Quantitative evaluation of testicular biopsy in patients with hydrocele using stereological formulae used by us has not been reported earlier. Also it is necessary in view of marked variations in the histology of the testis to use the patients own opposite testis as control.


   Conclusions Top


Large and tense vaginal hydroceles significantly affect the morphology and histology of the testis. The testis ap­pears to protect itself against an increased ambient pres­sure by a thickening of the tunica albugenia. However, this is not sufficient to protect the seminiferous tubules form distortion. There is no relationship between any of these alterations and the size of the duration of hydrocele. It is not known at present whether these changes revert to normal with treatment of the hydrocele as ethical consid­erations preclude a repeat testicular biopsy sometime af­ter treatment of the hydrocele for recording the fact. However, the fact that such changes occur is a strong ar­gument for advising surgical treatment of all hydroceles particularly in men in the reproductive age group.

 
   References Top

1.Bhatnagar BNS, Dube B, Shukla AP. Testicular histology in tropi­cal vaginal hydroceles. Int Surg 1970; 57:167-70.  Back to cited text no. 1    
2.Krahn HD, Tessler AN, Hotchhiss RS. Studies of the effect of hy­drocele upon scrotal temperature, pressure and testicular morphol­ogy. Fertil Steril 1963; 14:226-28.  Back to cited text no. 2    
3.Shiu MH, Van Langenberg A. Adult vaginal hydrocele in Hongkong, a study of 25 cases. J R Coll Surg Edinb 1967; 14:56-61.  Back to cited text no. 3    
4.Dandapat MC, Padhi NC, Patra AP. Effect of hydrocele on testis and spermatogenesis. Br J Surg 1990; 77: 1293-94.  Back to cited text no. 4  [PUBMED]  
5.Dedhia MC, Rao SS, Prabhu SR. Role of filariasis in the etiology of idiopathic hydroceles. Indian J Surg 1982; 44: 161-68  Back to cited text no. 5    
6.Scott AR, Hall Ad, Beepole PA, Crawford JD. Accuracy and repro­ducibility of clinical measures of testicular volume. J Pediatr 1987; 110: 914-16.  Back to cited text no. 6    
7.Hans E, Hyde DM. An elementary introduction to sterology (quan­titative microscopy) Am J Anat 1980; 159: 412.  Back to cited text no. 7    
8.Andres TE, Trainer TD, lapenas DT. Small vessel alterations in the testis of infertile men with varicocele. Am J Clin Pathol 1981; 76: 378-84.  Back to cited text no. 8    
9.Etriby A, Grigis SM, Henowy H, Ibrahim AA. Testicular changes in subfertile men with varicocele. Fertil Steril 1967; 18: 666-71.  Back to cited text no. 9    
10.Jones MA, Gregorly HS, Trainer TD. The adolescent varicocele - a histopathologic study of 13 testicular biopsies. Am J Clin Pathol 1988; 89: 321-28.  Back to cited text no. 10    
11.Saifee IA, Kukreti SC, Godbole VK. Effect of vaginal hydrocele on spermatogenesis. Indian J Surg 1980; 42: 130-33.  Back to cited text no. 11    


    Figures

  [Figure - 1], [Figure - 2]
 
 
    Tables

  [Table - 1], [Table - 2], [Table - 3]



 

Top
Print this article  Email this article
Previous article Next article

    

 
   Search
 
   Next article
   Previous article 
   Table of Contents
  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    [PDF Not available] *
    Citation Manager
    Access Statistics
    Reader Comments
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
    Introduction
    Patients and Methods
    Results
    Discussion
    Conclusions
    References
    Article Figures
    Article Tables

 Article Access Statistics
    Viewed5320    
    Printed136    
    Emailed1    
    PDF Downloaded0    
    Comments [Add]    

Recommend this journal

HEALTHWARE INDIA