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Year : 1996  |  Volume : 12  |  Issue : 1  |  Page : 57-58

Bone morphogenetic protein-6 expression in normal and malignant prostate


Department of Urology, Sri Venkateswara Institute of Medical Sciences, Tirupati, India

Correspondence Address:
H SGT Rao
Department of Urology, Sri Venkateswara Institute of Medical Sciences, Tirupati
India
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Source of Support: None, Conflict of Interest: None


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This study was conducted to elucidate the bone morphogenetic protein-6 (BMP-6) mRNA and protein expression in normal and malignant rat and human prostatic tissues. Normal ventral prostate was obtained from Copenhagen rats. Malignant rat prostate was Dunning tumour procured by the subcutaneous implantation of tumour cells in the flank of male Copenhagen rats. Human tissue was obtained immediately after surgery and inspected by a pathologist, and both normal and malignant tissue samples were obtained. For the purpose of immunohistochemistry, the prostatic tissue was fixed in 1 percent paraformaldehyde. For preparation of RNA, both rat and human prostate tissues were flash-frozen in liquid nitrogen and stored at 70 degree C. RNA was prepared by a lithium chloride/urea method. To investigate the effect of androgen withdrawal on BMP-6 expression, 12 adult male Copenhagen rats (300 g) were castrated. The concentration of BMP-6 mRNA was estimated by a reverse transcriptase- polymerase chain reaction (RT-PCRO). Over two Affinity-purified rabbit polyclonal antibody was used to detect BMP-6 protein immunohistochemically. Over three The levels of BMP-6 mRNA and protein were similar for normal and malignant rat prostate, regardless of the metastatic potential. Castration had no apparent effect on BMP-6 production in rat normal ventral prostate. BMP-6 mRNA and protein were also produced by normal and neoplastic human prostate cancer (clinical specimens and human carcinoma cell lines DU-145 and PC 3). BMP-6 mRNA and protein expression, in contradistinction to the adenocarcinoma cell line of rat, was higher in human prostate cancer as compared to the adjacent normal prostate. Tumours with Gleason score 6 or more had a greater BMP-6 immunostaining than the lower grade tumours, viz., those with Gleason score of 4 or less.


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