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Year : 1996  |  Volume : 12  |  Issue : 1  |  Page : 26-30

Successful pregnancy following microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection


Department of Infertility management and assisted reproduction, Jaslok Hospital, Mumbai

Correspondence Address:
F R Parikh
Department of Infertility management and assisted reproduction, Jaslok Hospital, Mumbai

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Source of Support: None, Conflict of Interest: None


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Between June 1995 and November 1995, Micro-surgical Epididymal Sperm Aspiration (MESA), and Intracytoplasmic Sperm Injection (ICSI) was performed on 9 patients (10 cycles) who had congenital absence of the vas deferens (n=5), failed vaso-epididymostomy (n=2) or other irreparable obstructions (n-2). In two patients no epididymal spermatozoa were recovered and so testicular sperm extraction (TESE) was carried out. In one cycle frozen-thawed epididymal spermatozoa was used. A total of 75 oocytes were retrieved (7.5 oocytes/cycle) of which 69 oocytes were micromanipulated. The fertilization rate was 65.2 percent and the cleavage rate was 95.5 percent. Maturation arrest at the pronuclear stage was observed in 4.5 percent. The damage rate was 4.3 percent. Assisted hatching (Partial Zona Dissection) was carried out on 43 embryos. 4.3 embryos were transferred per cycle. The pregnancy rate per cycle was 10 percent and per patient was 11.2 percent. MESA and TESE in combination with ICSI is the treatment of choice for men with obstructive azoospermic conditions. This paper reports the first pregnancy in India by the combined technique of Microsurgical Epididymal Sperm Aspiration and Intracytoplasmic Sperm Injection.


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